FitC: excitation light 10% intensity; 400ms exposure time

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Play, download and edit the free video FitC: excitation light 10% intensity; 400ms exposure time with Nanolive imaging on live pre-adipocyte cell.

This video shows the effect of excitation light at 10% intensity, 400ms exposure time and without fluorescent marker.
Increasing exposure time from 100ms to 400ms led to more serious cell damages. At 10% intensity and 400ms exposure time, the first sign of phototoxicity was the progressive slowdown of mitochondrial dynamics until an almost complete stop of mitochondrial movement reached after 30 min of acquisition, mitochondrial swelling was also observed in one replicate. This dynamic effect is obvious when looking at the full movie.
Imaging regimen: 1-hour acquisition at a frequency of 1 image every 6 seconds.

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